Human DPPIV/CD26 ELISA Kit

$350.00$450.00

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TARGETSDPP4
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity3.04 pg/mL
Range15.63 pg/mL – 1000 pg/mL
Assay Time3.5 h
Recovery95% – 110%
Average Recovery1.02
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity3.04 pg/mL
Range15.63 pg/mL – 1000 pg/mL
Assay Time3.5 h
Recovery95% – 110%
Average Recovery1.02
Intra Precision3.7%-5.5%
Inter-Precision3.9% – 5.4%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-DPPIV/CD26A monoclonal antibody
Human DPPIV/CD26A freeze-dried standard
DPPIV/CD26A detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human DPPIV antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, DPPIV present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of DPPIV in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

DPP4

DPP4 Target Infomation Overview

  • Target Symbol: DPP4, dipeptidyl peptidase 4
  • Gene Groups: CD molecules; DASH family
  • Alias: DPPIV
  • Previous Names: CD26; ADCP2
  • Alias Names: dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2); adenosine deaminase complexing protein 2; dipeptidyl-peptidase 4

DPP4, dipeptidyl peptidase 4 Target Infomation by Species

HumanMouseRat

Human DPP4 Target Information

  • Target Symbol: DPP4, dipeptidyl peptidase 4
  • Alias:
    • ADABP
    • ADCP-2
    • ADCP2
    • adenosine deaminase complexing protein 2
    • CD26
    • dipeptidyl peptidase IV
    • dipeptidyl-peptidase 4
    • dipeptidyl-peptidase 4 (CD26, adenosine deaminase complexing protein 2)
    • dipeptidylpeptidase 4
    • dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2)
    • DPP IV
    • DPPIV
    • Gly-Pro naphthylamidase
    • Post-proline dipeptidyl aminopeptidase IV
    • T-cell activation antigen CD26
    • TP103
    • Xaa-Pro-dipeptidylaminopeptidase
  • NCBI_Gene: 1803
  • UniProtKB: P27487

Human DPP4 Predicted Functions

Enables several functions, including chemorepellent activity; peptidase activity; and protein homodimerization activity. Involved in several processes, including negative regulation of extracellular matrix disassembly; negative regulation of neutrophil chemotaxis; and regulation of cell-cell adhesion. Located in several cellular components, including apical plasma membrane; endocytic vesicle; and lamellipodium. Implicated in Middle East respiratory syndrome and type 2 diabetes mellitus.

Mouse Dpp4 Target Information

Mouse Dpp4 Predicted Functions

Predicted to enable several functions, including chemorepellent activity; peptidase activity; and protein homodimerization activity. Acts upstream of or within behavioral fear response; locomotory exploration behavior; and psychomotor behavior. Located in intercellular canaliculus. Is expressed in several structures, including genitourinary system; inner ear; intraembryonic coelom; liver; and primitive heart tube. Used to study Middle East respiratory syndrome. Human ortholog(s) of this gene implicated in Middle East respiratory syndrome and type 2 diabetes mellitus. Orthologous to human DPP4 (dipeptidyl peptidase 4).

Rat Dpp4 Target Information

Rat Dpp4 Predicted Functions

Enables several functions, including collagen binding activity; dipeptidyl-peptidase activity; and identical protein binding activity. Involved in several processes, including B-1a B cell differentiation; proteolysis; and regulation of lymphocyte mediated immunity. Located in several cellular components, including Golgi apparatus; cell surface; and endoplasmic reticulum. Human ortholog(s) of this gene implicated in Middle East respiratory syndrome and type 2 diabetes mellitus. Orthologous to human DPP4 (dipeptidyl peptidase 4).

DPP4 Target News

    Obesity Diabetes

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