Human IL-11 ELISA Kit

$350.00$450.00

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TARGETSIL11
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample VolumeSerum, plasma: 20 μL;cell culture supernatant: 100 μL
Sensitivity5.01 pg/mL
Range62.5 pg/mL – 4000 pg/mL
Assay Time3.5 h
Recovery84% – 118%
Average Recovery0.99
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample VolumeSerum, plasma: 20 μL;cell culture supernatant: 100 μL
Sensitivity5.01 pg/mL
Range62.5 pg/mL – 4000 pg/mL
Assay Time3.5 h
Recovery84% – 118%
Average Recovery0.99
Intra Precision5.6% – 8.3%
Inter-Precision4.4% – 7.1%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-11 monoclonal antibody
Human IL-11 freeze-dried standard
IL-11 detect Antibody
Standard Diluent
HRP-labeled streptavidin
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IL-11 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-11 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-11 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

IL11

IL11 Target Infomation Overview

  • Target Symbol: IL11, interleukin 11
  • Gene Groups: Interleukins; Interleukin 6 type cytokine family
  • Alias: IL-11; AGIF
  • Alias Names: adipogenesis inhibitory factor; oprelvekin

IL11, interleukin 11 Target Infomation by Species

HumanMouseRat

Human IL11 Target Information

Human IL11 Predicted Functions

Enables growth factor activity. Involved in positive regulation of cell population proliferation. Acts upstream of or within several processes, including negative regulation of hormone secretion; positive regulation of MAPK cascade; and positive regulation of protein phosphorylation. Predicted to be located in extracellular region. Predicted to be active in cytoplasm.

Mouse Il11 Target Information

Mouse Il11 Predicted Functions

Enables cytokine activity and interleukin-11 receptor binding activity. Acts upstream of or within positive regulation of cell population proliferation. Located in cytoplasm. Orthologous to human IL11 (interleukin 11).

Rat Il11 Target Information

Rat Il11 Predicted Functions

Predicted to enable cytokine activity; growth factor activity; and interleukin-11 receptor binding activity. Predicted to be involved in positive regulation of MAPK cascade and positive regulation of cell population proliferation. Predicted to act upstream of or within negative regulation of hormone secretion; positive regulation of protein phosphorylation; and positive regulation of transcription by RNA polymerase II. Predicted to be located in extracellular space. Predicted to be active in cytoplasm. Orthologous to human IL11 (interleukin 11).

IL11 Target News

    11.6
    IL11 signaling mediates piR-2158 suppression of cell stemness and angiogenesis in breast cancer
    Theranostics

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