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Human IL-11 ELISA Kit
$350.00 – $450.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | Serum, plasma: 20 μL；cell culture supernatant: 100 μL |
| Sensitivity | 5.01 pg/mL |
| Range | 62.5 pg/mL – 4000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 84% – 118% |
| Average Recovery | 0.99 |
| Intra Precision | 5.6% – 8.3% |
| Inter-Precision | 4.4% – 7.1% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-11 monoclonal antibody Human IL-11 freeze-dried standard IL-11 detect Antibody Standard Diluent HRP-labeled streptavidin Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IL-11 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-11 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-11 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
IL11
IL11 Target Infomation Overview
- Target Symbol: IL11, interleukin 11
- Gene Groups: Interleukins; Interleukin 6 type cytokine family
- Alias: IL-11; AGIF
- Alias Names: adipogenesis inhibitory factor; oprelvekin
IL11, interleukin 11 Target Infomation by Species
HumanMouseRat
Human IL11 Target Information
- Target Symbol: IL11, interleukin 11
- Alias:
- adipogenesis inhibitory factor
- AGIF
- IL-11
- interleukin-11
- oprelvekin
- NCBI_Gene: 3589
- UniProtKB: P20809
Human IL11 Predicted Functions
Enables growth factor activity. Involved in positive regulation of cell population proliferation. Acts upstream of or within several processes, including negative regulation of hormone secretion; positive regulation of MAPK cascade; and positive regulation of protein phosphorylation. Predicted to be located in extracellular region. Predicted to be active in cytoplasm.
Mouse Il11 Target Information
- Target Symbol: Il11, interleukin 11
- Alias:
- IL-11
- NCBI_Gene: 16156
Mouse Il11 Predicted Functions
Enables cytokine activity and interleukin-11 receptor binding activity. Acts upstream of or within positive regulation of cell population proliferation. Located in cytoplasm. Orthologous to human IL11 (interleukin 11).
Rat Il11 Target Information
- Target Symbol: Il11, interleukin 11
- Alias:
- IL-11
- interleukin-11
- NCBI_Gene: 171040
Rat Il11 Predicted Functions
Predicted to enable cytokine activity; growth factor activity; and interleukin-11 receptor binding activity. Predicted to be involved in positive regulation of MAPK cascade and positive regulation of cell population proliferation. Predicted to act upstream of or within negative regulation of hormone secretion; positive regulation of protein phosphorylation; and positive regulation of transcription by RNA polymerase II. Predicted to be located in extracellular space. Predicted to be active in cytoplasm. Orthologous to human IL11 (interleukin 11).
