Human KGF/FGF-7 ELISA Kit

$350.00$450.00

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TARGETSFGF7
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity4.05 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery80% – 120%
Average Recovery0.96
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity4.05 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery80% – 120%
Average Recovery0.96
Intra Precision3.6% – 4.4%
Inter-Precision2.1% – 3.6%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-KGF monoclonal antibody
Human KGF freeze-dried standard
KGF detect Antibody
Standard Diluent
HRP-labeled streptavidin
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human KGF antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, KGF present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, the TMB chromogenic substrate is added, and color development is carried out while avoiding light. The intensity of the color reaction is directly proportional to the concentration of KGF in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

FGF7

FGF7 Target Infomation Overview

  • Target Symbol: FGF7, fibroblast growth factor 7
  • Gene Groups: Receptor ligands; Fibroblast growth factor family
  • Alias: KGF
  • Alias Names: keratinocyte growth factor; fibroblast growth factor 7 (keratinocyte growth factor)

FGF7, fibroblast growth factor 7 Target Infomation by Species

HumanMouseRat

Human FGF7 Target Information

Human FGF7 Predicted Functions

Enables chemoattractant activity. Involved in several processes, including positive regulation of keratinocyte migration; positive regulation of keratinocyte proliferation; and protein localization to cell surface. Acts upstream of or within fibroblast growth factor receptor signaling pathway and positive regulation of epithelial cell proliferation involved in lung morphogenesis. Predicted to be located in extracellular region. Predicted to be active in cytoplasm and extracellular space. Biomarker of several diseases, including inflammatory bowel disease (multiple); leiomyoma; prostatic hypertrophy; reproductive organ cancer (multiple); and severe acute respiratory syndrome.

Mouse Fgf7 Target Information

Mouse Fgf7 Predicted Functions

Predicted to enable heparin binding activity; receptor ligand activity; and type 2 fibroblast growth factor receptor binding activity. Involved in positive regulation of transcription, DNA-templated. Acts upstream of or within morphogenesis of an epithelium; positive regulation of epithelial cell proliferation; and regulation of branching involved in salivary gland morphogenesis by mesenchymal-epithelial signaling. Located in Golgi apparatus and extracellular region. Is expressed in several structures, including alimentary system; brain; embryo mesenchyme; genitourinary system; and integumental system. Orthologous to human FGF7 (fibroblast growth factor 7).

Rat Fgf7 Target Information

Rat Fgf7 Predicted Functions

Enables growth factor activity; heparin binding activity; and type 2 fibroblast growth factor receptor binding activity. Involved in several processes, including ovarian follicle development; phosphatidylcholine biosynthetic process; and surfactant homeostasis. Located in extracellular space. Orthologous to human FGF7 (fibroblast growth factor 7).

FGF7 Target News

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