Human TL-1A/TNFSF15 ELISA Kit

$350.00$450.00

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TARGETSTNFSF15
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity3.09 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery86% – 97%
Average Recovery0.9
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity3.09 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery86% – 97%
Average Recovery0.9
Intra Precision4.1% – 4.9%
Inter-Precision2.7%-5.6%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-TL-1A monoclonal antibody
Human TL-1A freeze-dried standard
Human TL-1A detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human TL-1A antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the TL-1A present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of TL-1A in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

TNFSF15

TNFSF15 Target Infomation Overview

  • Target Symbol: TNFSF15, TNF superfamily member 15
  • Gene Groups: Tumor necrosis factor superfamily
  • Alias: TL1; VEGI; TL1A; VEGI192A; MGC129934; MGC129935
  • Alias Names: vascular endothelial cell growth inhibitor; TNF superfamily ligand TL1A; TNF ligand-related molecule 1; vascular endothelial growth inhibitor-192A; tumor necrosis factor (ligand) superfamily, member 15

TNFSF15, TNF superfamily member 15 Target Infomation by Species

HumanMouseRat

Human TNFSF15 Target Information

  • Target Symbol: TNFSF15, TNF superfamily member 15
  • Alias:
    • MGC129934
    • MGC129935
    • TL1
    • TL1A
    • TNF ligand-related molecule 1
    • TNF superfamily ligand TL1A
    • TNLG1B
    • tumor necrosis factor (ligand) superfamily member 15
    • tumor necrosis factor (ligand) superfamily, member 15
    • tumor necrosis factor ligand 1B
    • tumor necrosis factor ligand superfamily member 15
    • tumor necrosis factor superfamily member 15
    • vascular endothelial cell growth inhibitor
    • vascular endothelial growth inhibitor-192A
    • VEGI
    • VEGI192A
  • NCBI_Gene: 9966
  • UniProtKB: O95150

Human TNFSF15 Predicted Functions

Predicted to enable signaling receptor binding activity. Acts upstream of or within activation of NF-kappaB-inducing kinase activity and activation of cysteine-type endopeptidase activity involved in apoptotic process. Located in plasma membrane.

Mouse Tnfsf15 Target Information

Mouse Tnfsf15 Predicted Functions

Predicted to enable death receptor binding activity. Predicted to be involved in activation of cysteine-type endopeptidase activity involved in apoptotic process and positive regulation of cytokine production. Predicted to act upstream of or within activation of NF-kappaB-inducing kinase activity. Predicted to be located in plasma membrane. Used to study inflammatory bowel disease 16. Orthologous to human TNFSF15 (TNF superfamily member 15).

Rat Tnfsf15 Target Information

  • Target Symbol: Tnfsf15, TNF superfamily member 15
  • Alias:
    • TNF ligand-related molecule 1
    • Tnlg1b
    • tumor necrosis factor (ligand) superfamily, member 15
    • tumor necrosis factor ligand 1b
    • tumor necrosis factor ligand superfamily member 15
    • tumor necrosis factor superfamily member 15
    • vascular endothelial cell growth inhibitor
  • NCBI_Gene: 252878

Rat Tnfsf15 Predicted Functions

Enables death receptor binding activity. Involved in activation of cysteine-type endopeptidase activity involved in apoptotic process and positive regulation of cytokine production. Predicted to be located in plasma membrane. Orthologous to human TNFSF15 (TNF superfamily member 15).

TNFSF15 Target News

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