Human Transferrin/TF ELISA Kit

ELISA Kits

$350.00 – $450.00

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Datasheet

SDS File

TARGETS	TF
SPECIES	Human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	50 μL
Sensitivity	2.82 pg/mL
Range	0.94 ng/mL – 60 ng/mL
Assay Time	3.5 h
Recovery	90% – 118%
Average Recovery	1.09

Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	50 μL
Sensitivity	2.82 pg/mL
Range	0.94 ng/mL – 60 ng/mL
Assay Time	3.5 h
Recovery	90% – 118%
Average Recovery	1.09
Intra Precision	1.8% – 2.6%
Inter-Precision	1.9% – 3.6%
Platform	ELISA
Plate	Detachable 96-well plate
Size	96T/48T
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Transferrin/TF monoclonal antibody Human Transferrin/TF freeze-dried standard Transferrin/TF detect Antibody Standard Diluent HRP-labeled streptavidin Signal enhancer concentrate Signal enhancer diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human TF antibodies are precoated on a high-affinity ELISA plate. Standard samples and test samples are added to the wells of the ELISA plate for incubation. After washing to remove unbound substances, the detection antibody labeled with horseradish peroxidase is added for further incubation. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of TF in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Targets

TF

TF Target Infomation Overview

Target Symbol: TF, transferrin
Gene Groups: Transferrins
Alias: PRO1557; PRO2086
Alias Names: serotransferrin

TF, transferrin Target Infomation by Species

HumanMouseRat

Human TF Target Information

Target Symbol: TF, transferrin
Alias:
- beta-1 metal-binding globulin
- DKFZp781D0156
- epididymis secretory sperm binding protein Li 71p
- HEL-S-71p
- PRO1557
- PRO2086
- serotransferrin
- siderophilin
- TFQTL1
NCBI_Gene: 7018
UniProtKB: P02787

Human TF Predicted Functions

Enables ferrous iron binding activity; iron chaperone activity; and transferrin receptor binding activity. Involved in cellular response to iron ion; positive regulation of receptor-mediated endocytosis; and regulation of iron ion transport. Located in several cellular components, including basal plasma membrane; clathrin-coated pit; and cytoplasmic vesicle. Is extrinsic component of external side of plasma membrane. Part of HFE-transferrin receptor complex. Implicated in anemia; atransferrinemia; glucose metabolism disease (multiple); iron deficiency anemia; and restless legs syndrome. Biomarker of glomerulonephritis; hypertension; and membranous glomerulonephritis.

Mouse Trf Target Information

Target Symbol: Trf, transferrin
Alias:
- AI266983
- expressed sequence AI266983
- HP
- hpx
- hypotransferrinemia with hemochromatosis
- Tfn
NCBI_Gene: 22041

Mouse Trf Predicted Functions

Predicted to enable iron chaperone activity; iron ion binding activity; and transferrin receptor binding activity. Involved in several processes, including ERK1 and ERK2 cascade; osteoclast differentiation; and positive regulation of bone resorption. Acts upstream of or within SMAD protein signal transduction; iron ion transport; and response to bacterium. Located in clathrin-coated pit and cytoplasmic vesicle. Is expressed in several structures, including brain; liver; placenta; white fat; and yolk sac. Used to study atransferrinemia. Human ortholog(s) of this gene implicated in anemia; atransferrinemia; glucose metabolism disease (multiple); iron deficiency anemia; and restless legs syndrome. Orthologous to human TF (transferrin).

Rat Tf Target Information

Target Symbol: Tf, transferrin
Alias:
- beta-1 metal-binding globulin
- liver regeneration-related protein LRRG03
- MGC93500
- serotransferrin
- siderophilin
- Tfn
- Trf
NCBI_Gene: 24825
- UniprotKB: A0A0G2QC06
- UniprotKB: P12346

Rat Tf Predicted Functions

Enables ferric iron binding activity. Involved in several processes, including cellular response to cAMP; cellular response to follicle-stimulating hormone stimulus; and regulation of nervous system development. Located in basement membrane; cell tip; and extracellular space. Used to study hepatitis. Biomarker of anemia and obesity. Human ortholog(s) of this gene implicated in anemia; atransferrinemia; glucose metabolism disease (multiple); iron deficiency anemia; and restless legs syndrome. Orthologous to human TF (transferrin).