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Mouse Galectin-1 ELISA Kit
$350.00 – $450.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 100 μL(diluent) |
| Sensitivity | 1.11 pg/mL |
| Range | 31.25 pg/mL – 2000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 71% – 117% |
| Average Recovery | 0.98 |
| Intra Precision | 2.4% – 3.9% |
| Inter-Precision | 2.7%-4.1% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Galectin-1 monoclonal antibody Mouse Galectin-1 freeze-dried standard Mouse Galectin-1 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse Galectin-1 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, Galectin-1 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Galectin-1 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
LGALS1
LGALS1 Target Infomation Overview
- Target Symbol: LGALS1, galectin 1
- Gene Groups: Receptor ligands; Galectins
- Alias: GBP
- Alias Names: lectin, galactoside-binding, soluble, 1
LGALS1, galectin 1 Target Infomation by Species
HumanMouseRat
Human LGALS1 Target Information
- Target Symbol: LGALS1, galectin 1
- Alias:
- 14 kDa laminin-binding protein
- 14 kDa lectin
- beta-galactoside-binding lectin L-14-I
- beta-galactoside-binding protein 14kDa
- DKFZp686E23103
- gal-1
- GAL1
- galaptin
- galectin-1
- GBP
- HBL
- HLBP14
- HPL
- lactose-binding lectin 1
- lectin, galactoside binding soluble 1
- lectin, galactoside-binding, soluble, 1
- putative MAPK-activating protein PM12
- S-Lac lectin 1
- NCBI_Gene: 3956
- UniProtKB: P09382
Human LGALS1 Predicted Functions
Enables RNA binding activity. Involved in positive regulation of viral entry into host cell. Located in extracellular exosome. Colocalizes with collagen-containing extracellular matrix.
Mouse Lgals1 Target Information
- Target Symbol: Lgals1, lectin, galactose binding, soluble 1
- Alias:
- AA410090
- beta galactoside binding protein
- expressed sequence AA410090
- Gal-1
- Galbp
- galectin-1
- L14
- lactose binding soluble lectin, 14 kDa
- Lect14
- NCBI_Gene: 16852
Mouse Lgals1 Predicted Functions
Enables carbohydrate binding activity. Acts upstream of or within T cell costimulation; myoblast differentiation; and plasma cell differentiation. Located in extracellular space. Is expressed in several structures, including embryo mesenchyme; genitourinary system; integumental system; main olfactory bulb; and olfactory system. Orthologous to human LGALS1 (galectin 1).
Rat Lgals1 Target Information
- Target Symbol: Lgals1, galectin 1
- Alias:
- 14 kDa lectin
- beta-galactoside-binding lectin
- beta-galactoside-binding lectin L-14-I
- gal-1
- galaptin
- galectin-1
- lactose-binding lectin 1
- lectin, galactose binding, soluble 1
- lectin, galactoside-binding, soluble, 1
- RL 14.5
- S-Lac lectin 1
- NCBI_Gene: 56646
- UniProtKB: P11762
Rat Lgals1 Predicted Functions
Enables identical protein binding activity; lactose binding activity; and laminin binding activity. Involved in several processes, including cellular response to glucose stimulus; positive regulation of erythrocyte aggregation; and response to isolation stress. Located in cell surface; cytosol; and nucleus. Biomarker of B-cell lymphoma; extrahepatic cholestasis; and lipoid nephrosis. Orthologous to human LGALS1 (galectin 1).
