Mouse IGFBP-3 ELISA Kit

$350.00$450.00

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TARGETSIGFBP3
SPECIESMouse
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL(diluent)
Sensitivity1.42 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery83% – 110%
Average Recovery0.95
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL(diluent)
Sensitivity1.42 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery83% – 110%
Average Recovery0.95
Intra Precision1.9% – 3.2%
Inter-Precision3.0%-4.4%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IGFBP-3 monoclonal antibody
Mouse IGFBP-3 freeze-dried standard
Mouse IGFBP-3 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse IGFBP-3 antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the IGFBP-3 present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IGFBP-3 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

IGFBP3

IGFBP3 Target Infomation Overview

  • Target Symbol: IGFBP3, insulin like growth factor binding protein 3
  • Gene Groups: Insulin like growth factor binding proteins
  • Alias: IBP3; BP-53
  • Alias Names: growth hormone-dependent binding protein; acid stable subunit of the 140 K IGF complex; binding protein 53; binding protein 29; IGF-binding protein 3; insulin-like growth factor binding protein 3

IGFBP3, insulin like growth factor binding protein 3 Target Infomation by Species

HumanMouseRat

Human IGFBP3 Target Information

  • Target Symbol: IGFBP3, insulin like growth factor binding protein 3
  • Alias:
    • acid stable subunit of the 140 K IGF complex
    • binding protein 29
    • binding protein 53
    • BP-53
    • growth hormone-dependent binding protein
    • IBP-3
    • IBP3
    • IGF-binding protein 3
    • IGFBP-3
    • insulin-like growth factor binding protein 3
    • insulin-like growth factor-binding protein 3
  • NCBI_Gene: 3486
  • UniProtKB: P17936

Human IGFBP3 Predicted Functions

Enables insulin-like growth factor I binding activity and protein tyrosine phosphatase activator activity. Involved in several processes, including negative regulation of smooth muscle cell migration; negative regulation of smooth muscle cell proliferation; and positive regulation of myoblast differentiation. Acts upstream of or within negative regulation of cell population proliferation; negative regulation of protein phosphorylation; and protein phosphorylation. Located in extracellular space and nucleus. Part of insulin-like growth factor ternary complex. Implicated in several diseases, including Alzheimer’s disease; Turner syndrome; diabetes mellitus (multiple); prostate cancer; and transitional cell carcinoma. Biomarker of several diseases, including Barrett’s esophagus; Down syndrome; autoimmune disease (multiple); bone disease (multiple); and urinary system cancer (multiple).

Mouse Igfbp3 Target Information

Mouse Igfbp3 Predicted Functions

Enables fibronectin binding activity and insulin-like growth factor binding activity. Acts upstream of or within several processes, including osteoblast differentiation; positive regulation of signal transduction; and type B pancreatic cell proliferation. Located in extracellular space. Is expressed in several structures, including alimentary system; brain; early embryo; gonad; and limb. Human ortholog(s) of this gene implicated in several diseases, including Alzheimer’s disease; Turner syndrome; diabetes mellitus (multiple); prostate cancer; and transitional cell carcinoma. Orthologous to human IGFBP3 (insulin like growth factor binding protein 3).

Rat Igfbp3 Target Information

Rat Igfbp3 Predicted Functions

Enables insulin-like growth factor II binding activity. Involved in several processes, including cellular response to Thyroglobulin triiodothyronine; cellular response to estradiol stimulus; and response to peptide hormone. Located in extracellular space; heterochromatin; and platelet alpha granule. Part of insulin-like growth factor binary complex and insulin-like growth factor ternary complex. Used to study impotence. Biomarker of congenital diaphragmatic hernia; impotence; and obesity. Human ortholog(s) of this gene implicated in several diseases, including Alzheimer’s disease; Turner syndrome; diabetes mellitus (multiple); prostate cancer; and transitional cell carcinoma. Orthologous to human IGFBP3 (insulin like growth factor binding protein 3).

IGFBP3 Target News

    Obesity Diabetes
    Osteoporosis
    Tumor Marker

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