Mouse IL-9 ELISA Kit

$350.00$450.00

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TARGETSIL9
SPECIESMouse
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity3.59 pg/mL
Range125 pg/mL – 8000 pg/mL
Assay Time3.5 h
Recovery95% – 117%
Average Recovery1.08
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity3.59 pg/mL
Range125 pg/mL – 8000 pg/mL
Assay Time3.5 h
Recovery95% – 117%
Average Recovery1.08
Intra Precision2.5% – 3.7%
Inter-Precision2.8% – 4.6%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-9 monoclonal antibody
Mouse IL-9 freeze-dried standard
IL-9 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse IL-9 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-9 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-9 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

IL9

IL9 Target Infomation Overview

  • Target Symbol: IL9, interleukin 9
  • Gene Groups: Interleukins
  • Alias: IL-9; HP40; P40
  • Alias Names: p40 T-cell and mast cell growth factor; T-cell growth factor p40; p40 cytokine; homolog of mouse T cell and mast cell growth factor 40

IL9, interleukin 9 Target Infomation by Species

HumanMouseRat

Human IL9 Target Information

  • Target Symbol: IL9, interleukin 9
  • Alias:
    • cytokine P40
    • homolog of mouse T cell and mast cell growth factor 40
    • HP40
    • IL-9
    • interleukin-9
    • P40
    • p40 cytokine
    • p40 T-cell and mast cell growth factor
    • T-cell growth factor p40
  • NCBI_Gene: 3578
  • UniProtKB: P15248

Human IL9 Predicted Functions

Predicted to enable cytokine activity and interleukin-9 receptor binding activity. Predicted to be involved in positive regulation of interleukin-5 production. Predicted to act upstream of or within several processes, including B cell activation; regulation of cellular protein metabolic process; and regulation of receptor signaling pathway via JAK-STAT. Predicted to be located in extracellular region. Predicted to be active in extracellular space. Implicated in asthma and respiratory syncytial virus infectious disease. Biomarker of COVID-19; asthma; cystic fibrosis; lung disease (multiple); and rhinitis.

Mouse Il9 Target Information

Mouse Il9 Predicted Functions

Enables cytokine activity. Involved in positive regulation of interleukin-5 production. Acts upstream of or within several processes, including B cell activation; regulation of cellular protein metabolic process; and regulation of receptor signaling pathway via JAK-STAT. Located in extracellular space. Is expressed in gut; liver; thymus; and thymus primordium. Human ortholog(s) of this gene implicated in asthma and respiratory syncytial virus infectious disease. Orthologous to human IL9 (interleukin 9).

Rat Il9 Target Information

Rat Il9 Predicted Functions

Enables cytokine activity and interleukin-9 receptor binding activity. Involved in positive regulation of cell growth. Predicted to be active in extracellular space. Human ortholog(s) of this gene implicated in asthma and respiratory syncytial virus infectious disease. Orthologous to human IL9 (interleukin 9).

IL9 Target News

    5.4
    Baiheqingjin formula reduces inflammation in mice with asthma by inhibiting the PI3K/AKT/NF-κb signaling pathway
    JOURNAL OF ETHNOPHARMACOLOGY
    5.4
    Effects of total alkaloids from Alstonia scholaris (L.) R. Br. on ovalbumin-induced asthma mice
    JOURNAL OF ETHNOPHARMACOLOGY
    Allergic Reaction
    Lung Disease (Asthma & COPD)

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