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Mouse Ig Isotyping ELISA Kit
$350.00 – $450.00
| SPECIES | Mouse |
|---|---|
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
| Assay Time | 3.5 h |
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Assay Time | 3.5 h |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | Pre-coated ELISA plate detect Antibody Positive control 10×Assay Buffer Substrate TMB Stop Solution 20×Washing Buffer Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique. Specific monoclonal antibodies against mouse immunoglobulin subtypes are precoated on a high-affinity ELISA plate. The ELISA plate wells are added with test samples, positive controls, and HRP-conjugated detection antibodies. After incubation, the immunoglobulins present in the samples bind to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, TMB chromogenic substrate is added for color development while avoiding light. The reaction is terminated by adding a stop solution, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
