Mouse IL-18 ELISA Kit

$350.00$450.00

TARGETS IL18
SPECIES Mouse
Sample Type Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume Serum, plasma: 20 μL;cell culture supernatant: 100 μL
Sensitivity 0.93 pg/mL
Range 62.5 pg/mL – 4000 pg/mL
Assay Time 3.5 h
Recovery 80% – 119%
Average Recovery 0.98
Sample Type Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume Serum, plasma: 20 μL;cell culture supernatant: 100 μL
Sensitivity 0.93 pg/mL
Range 62.5 pg/mL – 4000 pg/mL
Assay Time 3.5 h
Recovery 80% – 119%
Average Recovery 0.98
Intra Precision 2.7% – 4.0%
Inter-Precision 2.4% – 3.7%
Platform ELISA
Plate Detachable 96-well plate
Size 96T/48T
Storage If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery 4℃ blue ice transportation
Components 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Mouse IL-18 monoclonal antibody
Mouse IL-18 freeze-dried standard
IL-18 detect Antibody
HRP-labeled streptavidin
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay Principle This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse IL-18 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and the detection antibody labeled with horseradish peroxidase are added to the wells of the ELISA plate. After incubation, IL-18 present in the samples binds to the solid-phase antibody and the detection antibody. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-18 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

IL18

IL18 Target Infomation Overview

  • Target Symbol: IL18, interleukin 18
  • Gene Groups: Interleukins
  • Alias: IGIF; IL1F4; IL-1g; IL-18
  • Alias Names: interferon-gamma-inducing factor; interleukin 18 (interferon-gamma-inducing factor)

IL18, interleukin 18 Target Infomation by Species

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Human IL18 Target Information

  • Target Symbol: IL18, interleukin 18
  • Alias:
    • iboctadekin
    • IFN-gamma-inducing factor
    • IGIF
    • IL-1 gamma
    • IL-18
    • IL-1g
    • IL1F4
    • interferon gamma-inducing factor
    • interleukin 18 (interferon-gamma-inducing factor)
    • interleukin-1 gamma
    • interleukin-18
    • MGC12320
  • NCBI_Gene: 3606
  • UniProtKB: Q14116

Human IL18 Predicted Functions

Enables cytokine activity and interleukin-18 receptor binding activity. Involved in several processes, including positive regulation of cell population proliferation; positive regulation of intracellular signal transduction; and positive regulation of macromolecule metabolic process. Located in extracellular space. Implicated in several diseases, including Kawasaki disease; allergic rhinitis; autoimmune disease (multiple); biliary atresia; and hepatitis B. Biomarker of several diseases, including autoimmune disease (multiple); cholestasis (multiple); liver disease (multiple); lung disease (multiple); and myositis (multiple).

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Mouse Il18 Target Information

Mouse Il18 Predicted Functions

Enables cytokine activity. Involved in lipid homeostasis; positive regulation of cell differentiation; and positive regulation of cold-induced thermogenesis. Acts upstream of or within several processes, including interleukin-18-mediated signaling pathway; positive regulation of NIK/NF-kappaB signaling; and positive regulation of interferon-gamma production. Located in extracellular space. Is expressed in several structures, including back skin; dorsal aorta; gut; lymphatic system; and reproductive system. Used to study atopic dermatitis. Human ortholog(s) of this gene implicated in several diseases, including Kawasaki disease; allergic rhinitis; autoimmune disease (multiple); biliary atresia; and hepatitis B. Orthologous to human IL18 (interleukin 18).

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Rat Il18 Target Information

Rat Il18 Predicted Functions

Predicted to enable cytokine activity and interleukin-18 receptor binding activity. Involved in several processes, including cellular response to hormone stimulus; positive regulation of cell population proliferation; and positive regulation of cytokine production. Located in apical plasma membrane; cytoplasm; and extracellular space. Used to study brain edema; fatty liver disease; heart disease; status epilepticus; and vasculitis. Biomarker of several diseases, including cardiac arrest; ischemia (multiple); lung disease (multiple); perinatal necrotizing enterocolitis; and urinary tract infection. Human ortholog(s) of this gene implicated in several diseases, including Kawasaki disease; allergic rhinitis; autoimmune disease (multiple); biliary atresia; and hepatitis B. Orthologous to human IL18 (interleukin 18).

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IL18 Target News

[catlist tags=”il18″ template=targetnews thumbnail=yes thumbnail_class=”related-post-media clr” thumbnail_size=833]

9.3
Aberrant copper metabolism and hepatic inflammation cause neurological manifestations in a mouse model of Wilson’s disease
Journal of Neuroinflammation
4.3
EGB761 ameliorates mild cognitive impairment by inhibiting the pyroptosis and apoptosis in both in vivo and in vitro experiments
ARCHIV DER PHARMAZIE
3.4
Silencing of TXNIP attenuates oxidative stress injury in HEI-OC1 by inhibiting the activation of NLRP3 and NF-κB
Heliyon
13.2
Construction of drug-free sodium bicarbonate nanoparticles with high water-tolerance for gas therapy to selectively induce non-apoptotic death of cancer cells
Nano Today
6.8
Discovery of a Covalent Inhibitor of Pro-Caspase-1 Zymogen Blocking NLRP3 Inflammasome Activation and Pyroptosis
JOURNAL OF MEDICINAL CHEMISTRY
4.8
Pectolinarigenin targeting FGFR3 alleviates osteoarthritis progression by regulating the NF-κB/NLRP3 inflammasome pyroptotic pathway
INTERNATIONAL IMMUNOPHARMACOLOGY
6.7
Guizhitongluo Tablet inhibits atherosclerosis and foam cell formation through regulating Piezo1/NLRP3 mediated macrophage pyroptosis
PHYTOMEDICINE
5.3
Chloranthalactone B covalently binds to the NACHT domain of NLRP3 to attenuate NLRP3-driven inflammation
BIOCHEMICAL PHARMACOLOGY
4.7
Microbiome Metabolomic Analysis of the Anxiolytic Effect of Baihe Dihuang Decoction in a Rat Model of Chronic Restraint Stress
Drug Design Development and Therapy
4.4
Li-Hong Tang alleviates dextran sodium sulfate-induced colitis by regulating NRF2/HO-1 signaling pathway and gut microbiota
Frontiers in Pharmacology
2.9
TMEM79 Ameliorates Cerebral Ischemia/Reperfusion Injury Through Regulating Inflammation and Oxidative Stress via the Nrf2/NLRP3 Pathway
IMMUNOLOGICAL INVESTIGATIONS
4.8
Cortistatin protects against septic cardiomyopathy by inhibiting cardiomyocyte pyroptosis through the SSTR2-AMPK-NLRP3 pathway
INTERNATIONAL IMMUNOPHARMACOLOGY
8.5
Growth differentiation factor 11 regulates high glucose-induced cardiomyocyte pyroptosis and diabetic cardiomyopathy by inhibiting inflammasome activation
Cardiovascular Diabetology
5.3
Intracellular and extracellular Cyclophilin a promote cardiac fibrosis through TGF-β signaling in response to angiotensin Ⅱ
BIOCHEMICAL PHARMACOLOGY
6.8
Novel Isoalantolactone-Based Derivatives as Potent NLRP3 Inflammasome Inhibitors: Design, Synthesis, and Biological Characterization
JOURNAL OF MEDICINAL CHEMISTRY
4.8
The Scutellaria-Coptis herb couple and its active small-molecule ingredient wogonoside alleviate cytokine storm by regulating the CD39/NLRP3/GSDMD signaling pathway
JOURNAL OF ETHNOPHARMACOLOGY
Allergic Reaction
Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
Lung Disease (Asthma & COPD)
Multiple Sclerosis
Nervous System Diseases (Depression & Alzheimer & Epilepsy & Parkinson)
Obesity Diabetes
Osteoarthrosis & Rheumatoid Arthritis
Sepsis
Systemic Lupus Erythematosus
Transplantation

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